☆ 4.7 Review

Proteolytic Biosensors with Functional Nanomaterials: Current Approaches and Future Challenges

BIOSENSORS-BASEL (2023)

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Summary: In this study, a label-free peptide substrate was used to induce the aggregation of AuNPs for visual analysis of Mpro activity. Co-assembly of AuNPs and peptide on an electrode surface allowed for the development of a simple and sensitive electrochemical method for detecting Mpro in serum samples.

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Identification of novel anti-cancer agents, applying in silico method for SENP1 protease inhibition

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Summary: In this study, a new method for measuring SARS-CoV-2 infection is described which is simpler and faster than existing methods. It relies on the reaction between a specific protease enzyme produced by the virus and an engineered protein biosensor to detect infection. This method can be used to detect different variants of SARS-CoV-2 and quantify neutralising antibodies.

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A Photoacoustic-Fluorescent Imaging Probe for Proteolytic Gingipains Expressed by Porphyromonas gingivalis

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LANGMUIR (2022)

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Thiol-sensitive probe enables dynamic electrochemical assembly of serum protein for detecting SARS-Cov-2 marker protease in clinical samples

Kai Zhang et al.

Summary: A low-cost and simple operation virus protein serum assay has been proposed, targeting the main protease of the virus through a short probe mimicking its substrate. Successful detection of the virus marker protein in the serum of infected patients encourages further development of this method for clinical practice.

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Modular Combination of Proteolysis-Responsive Transcription and Spherical Nucleic Acids for Smartphone-Based Colorimetric Detection of Protease Biomarkers

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