期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 10, 页码 1935-1944出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600010
关键词
Central composite design; Microextraction; Solidification; Urine; Valsartan
资金
- Yazd University
- Behbahan Khatam Alanbia University of Technology
In this work, a fast, easy, and efficient dispersive liquid-liquid microextraction method based on solidification of floating organic drop followed by high-performance liquid chromatography with UV detection was developed for the separation/preconcentration and determination of the drug valsartan. Experimental design was applied for the optimization of the effective variables (such as volume of extracting and dispersing solvents, ionic strength, and pH) on the extraction efficiency of valsartan from urine samples. The optimized values were 250.0 mu L ethanol, 65.0 mu L 1-dodecanol, 4.0% w/v NaCl, pH 3.8, 1.0 min extraction time, and 4.0 min centrifugation at 4000 rpm min(-1). The linear response (r(2) = 0.997) was obtained in the range of 0.013-10.0 mu g mL(-1) with a limit of detection of 4.0 ng mL(-1) and relative standard deviations of less than 5.0 % (n = 6).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据