期刊
CELL RESEARCH
卷 26, 期 2, 页码 239-253出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2015.152
关键词
NudC; actin dynamics; ciliogenesis; cofilin 1; zebrafish development
类别
资金
- Ministry of Science and Technology of China [2012CB945004, 2013CB945603, 2011CBA01001]
- National Natural Science Foundation of China [31125017, 31190063, 31100975, 31571446, 31471259]
- Ministry of Education of China [20110101110103]
- Natural Scientific Foundation of Zhejiang Province, China [LY14C070001, LY14C070002]
- 111 Project [B13026]
- Department of Science and Technology of Zhejiang Province [2012C37089, 2013TD13]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents
Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates actin cytoskeleton and ciliogenesis by stabilizing cofilin 1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据