期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 1, 页码 21-37出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500634
关键词
Interfaces; Metabonomics; Orthogonality; Traditional Chinese medicine; Two-dimensional liquid chromatography
资金
- Qing Lan Project of Jiangsu Province
- Six Talent Peak Project of Jiangsu Province
- Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Natural Science Foundation of Jiangsu Province [BK2011211]
Two-dimensional liquid chromatography has become an attractive analytical tool for the separation of complex samples due to its enhanced selectivity, peak capacity, and resolution compared with one-dimensional liquid chromatography. Recently, more attention has been drawn on the application of this separation technique in studies concerning traditional Chinese medicines, metabonomes, proteomes, and other complex mixtures. In this review, we aim to examine the application of two-dimensional liquid chromatography in traditional Chinese medicine analysis and metabonomic investigation. The classification and evaluation indexes were first introduced. Then, various switching methods were summarized when used in an on-line two-dimensional liquid chromatography system. Finally, the applications of this separation technique in traditional Chinese medicine analysis and metabonomic investigation were discussed on the basis of specific studies.
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