期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 18, 页码 3544-3549出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600375
关键词
Anion exchange chromatography; Minicircle DNA; Monoliths; Parental plasmids; Recombinant DNA technology
资金
- FCT, the Portuguese Foundation for Science and Technology [UID/Multi/00709/2013, SFRH/BPD/102716/2014]
Minicircle DNA is a new biotechnological product with beneficial therapeutic perspectives for gene therapy because it is constituted only by the eukaryotic transcription unit. These features improve minicircle DNA safety and increase its therapeutic effect. However, being a recently developed product, there is a need to establish efficient purification methodologies, enabling the recovery of the supercoiled minicircle DNA isoform. Thus, this work describes the minicircle DNA purification using an anion exchange monolithic support. The results show that with this column it is possible to achieve a good selectivity, which allows the isolation of the supercoiled minicircle DNA isoform from impurities. Overall, this study shows a promising approach to obtain the minicircle DNA sample with adequate quality for future therapeutic applications.
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