期刊
CELL RESEARCH
卷 25, 期 7, 页码 864-876出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2015.77
关键词
HITS-CLIP/CLIP-seq; RNA-binding protein; alternative polyadenylation; APA; flowering; FCA
类别
资金
- National Basic Research Program of China [2014CB943400, 2011CB915400, 2013CB967303]
- National Natural Science Foundation of China [31330020, 31210103901, 31171184, 90919033, 31200900]
- Transgenic Project [2014ZX08010-002]
- Chinese Academy of Sciences [KSCX2-EW-Q-24]
- State Key Laboratory of Plant Genomics [2014B0227-01, 2015B0129-01]
Alternative polyadenylation (APA) is a widespread mechanism for gene regulation and has been implicated in flowering, but the molecular basis governing the choice of a specific poly(A) site during the vegetative-to-reproductive growth transition remains unclear. Here we characterize HLP1, an hnRNP A/B protein as a novel regulator for pre-mRNA 3'-end processing in Arabidopsis. Genetic analysis reveals that HLP1 suppresses Flowering Locus C (FLC), a key repressor of flowering in Arabidopsis. Genome-wide mapping of HLP1-RNA interactions indicates that HLP1 binds preferentially to A-rich and U-rich elements around cleavage and polyadenylation sites, implicating its role in 3'-end formation. We show HLP1 is significantly enriched at transcripts involved in RNA metabolism and flowering. Comprehensive profiling of the poly(A) site usage reveals that HLP1 mutations cause thousands of poly(A) site shifts. A distal-to-proximal poly(A) site shift in the flowering regulator FCA, a direct target of HLP1, leads to upregulation of FLC and delayed flowering. Our results elucidate that HLP1 is a novel factor involved in 3'-end processing and controls reproductive timing via targeting APA.
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