Timed daily access to a running-wheel synchronizes circadian rhythms and promotes stable rhythms in animals with impaired neuropeptide signaling. The transcriptome of the brain clock showed extensive dysregulation in these animals, but the molecular programs in the liver and lung were partially intact. Furthermore, exercise could correct behavioral abnormalities in circadian rhythms without causing large scale alterations in the brain clock.
Timed daily access to a running-wheel (scheduled voluntary exercise; SVE) synchronizes rodent circadian rhythms and promotes stable, 24h rhythms in animals with genetically targeted impairment of neuropeptide signaling (Vipr2(-/-) mice). Here we used RNA-seq and/or qRT-PCR to assess how this neuropeptide signaling impairment as well as SVE shapes molecular programs in the brain clock (suprachiasmatic nuclei; SCN) and peripheral tissues (liver and lung). Compared to Vipr2(+/+) animals, the SCN transcriptome of Vipr2(-/-) mice showed extensive dysregulation which included core clock components, transcription factors, and neurochemicals. Furthermore, although SVE stabilized behavioral rhythms in these animals, the SCN transcriptome remained dysregulated. The molecular programs in the lung and liver of Vipr2(-/-) mice were partially intact, although their response to SVE differed to that of these peripheral tissues in the Vipr2(+/+) mice. These findings highlight that SVE can correct behavioral abnormalities in circadian rhythms without causing large scale alterations to the SCN transcriptome.
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