4.7 Review

Review Mechanobiology: A landscape for reinterpreting stem cell heterogeneity and regenerative potential in diseased tissues

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Summary: Organoids are a novel three-dimensional stem cell culture system that mimics the complexity of organ/tissue structure. The balance of microenvironmental components is crucial for successful development, and mechanical forces play a key role in stem cell specification process towards organoid formation.

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Summary: This article discusses the complexity of signals controlling the onset of biological shapes and functions, emphasizing the role of physical forces in cellular decisions. It presents the strong connection between biological patterns and the vibrational nature of physical energies, and describes biological dynamics as informational processes. The article highlights the significance of cytoskeletal elements in shaping biological identity and explores the potential of utilizing physical energies for tissue regeneration.

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Cytochalasin B Modulates Nanomechanical Patterning and Fate in Human Adipose-Derived Stem Cells

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Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum

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Summary: Research has found that calcium signaling is involved in mechanosensing during Dictyostelium development, including cell motility and morphogenesis, through different molecular mechanisms.

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Functional heterogeneity of mesenchymal stem cells from natural niches to culture conditions: implications for further clinical uses

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Summary: Studies have shown that mesenchymal stem cells (MSC) have therapeutic potential in various organs and tissues. However, their functional heterogeneity poses a challenge in transferring their capabilities to clinical applications. Proper selection of MSC based on their functional potential and improving their features in the artificial niche are essential for personalized and precise medicine.

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Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation

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Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.

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Role of the extracellular matrix and YAP/TAZ in cell reprogramming

Lan Liu et al.

Summary: Stem cells are crucial in regenerative medicine and cell therapy, and mechanical signals in the cellular microenvironment play an important role in inducing reprogramming of somatic cells into stem cells. These signals may activate physical and chemical pathways, with the ENH protein likely playing a key role in connecting and regulating these pathways. This illustrates a new strategy for inducing and regulating reprogramming in vitro through physical processes related to local mechanical forces.

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Fueling the cytoskeleton - links between cell metabolism and actin remodeling

Gillian DeWane et al.

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Two mechanisms drive pronuclear migration in mouse zygotes

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Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion

Chrystian Junqueira Alves et al.

Summary: The guidance receptor Plexin-B2 plays a significant role in organizing actomyosin network and adhesion complexes during multicellular development of human embryonic stem cells and neuroprogenitor cells. Manipulations of Plexin-B2 affect actomyosin contractility, leading to changes in cell stiffness and cytoskeletal tension, as well as cell-cell and cell-matrix adhesion. The study establishes the importance of collective cell mechanics in governing stem cell physiology and tissue morphogenesis.

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The extracellular matrix protein agrin is essential for epicardial epithelial-to-mesenchymal transition during heart development

Xin Sun et al.

Summary: During heart development, epicardial cells undergo epithelial-mesenchymal transition (EMT) and migrate into myocardium, supporting organ growth. Agrin, a proteoglycan associated with extracellular matrix, is crucial for regulating epicardial EMT and basement membrane integrity, impacting heart development significantly.

DEVELOPMENT (2021)

Article Multidisciplinary Sciences

Actuation enhances patterning in human neural tube organoids

Abdel Rahman Abdel Fattah et al.

Summary: The complex spatial organization of tissues is achieved through a combination of gene regulatory networks, cell-cell communication, and mechanical forces. Applying active mechanical forces to developing neural tubes can increase growth and enhance patterning in organoids. Organoids respond to mechanical regulation through a temporally restricted competence window, mediated by matrix stiffness, cytoskeleton contractility, and planar cell polarity.

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Mesenchymal Stromal Cell-Produced Components of Extracellular Matrix Potentiate Multipotent Stem Cell Response to Differentiation Stimuli

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Single Cell Mechanotype and Associated Molecular Changes in Urothelial Cell Transformation and Progression

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Hydraulic control of mammalian embryo size and cell fate

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Two distinct actin waves correlated with turns-and-runs of crawling microglia

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Shock wave treatment after hindlimb ischaemia results in increased perfusion and M2 macrophage presence

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Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors

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Shockwaves prevent from heart failure after acute myocardial ischaemia via RNA/protein complexes

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Actomyosin pulsation and flows in an active elastomer with turnover and network remodeling

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Toll-like receptor 3 signalling mediates angiogenic response upon shock wave treatment of ischaemic muscle

Johannes Holfeld et al.

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Asymmetric division of contractile domains couples cell positioning and fate specification

Jean-Leon Maitre et al.

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Epicardial shock-wave therapy improves ventricular function in a porcine model of ischaemic heart disease

Johannes Holfeld et al.

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Environmental physical cues determine the lineage specification of mesenchymal stem cells

Chao Huang et al.

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A guide to mechanobiology: Where biology and physics meet

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Bioelectric memory: modeling resting potential bistability in amphibian embryos and mammalian cells

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Pluripotent Stem Cells Derived From Mouse and Human White Mature Adipocytes

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Multi-level memory-switching properties of a single brain microtubule

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Cyclic strain dominates over microtopography in regulating cytoskeletal and focal adhesion remodeling of human mesenchymal stem cells

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Mechanical Loading Regulates NFATc1 and β-Catenin Signaling through a GSK3β Control Node

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Emergence of Patterned Stem Cell Differentiation Within Multicellular Structures

Sami Alom Ruiz et al.

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Nanomechanical analysis of cells from cancer patients

Sarah E. Cross et al.

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Adipogenesis is inhibited by brief, daily exposure to high-frequency, extremely low-magnitude mechanical signals

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