期刊
ZOOLOGICAL RESEARCH
卷 44, 期 1, 页码 63-77出版社
SCIENCE PRESS
DOI: 10.24272/j.issn.2095-8137.2022.167
关键词
Zebrafish; scd; Liver; Lipid homeostasis; Reproduction
类别
This study investigates the role of Stearyl coenzyme A desaturase (SCD) in lipid metabolism and sexual development using a zebrafish scd mutant. The mutant fish displayed increased levels of saturated fatty acids and decreased levels of monounsaturated fatty acids. They also exhibited abnormalities in body size, fat accumulation in the liver, hepatic mitochondrial dysfunction, and cell apoptosis. The mutant males showed defective mating behavior due to genital papillae defects but had functional mature sperm. Overexpression of scd rescued all the observed defects in the mutant fish.
Stearyl coenzyme A desaturase (SCD), also known as delta-9 desaturase, catalyzes the rate-limiting step in the formation of monounsaturated fatty acids. In mammals, depletion or inhibition of SCD activity generally leads to a decrease in triglycerides and cholesteryl esters. However, the endogenous role of scd in teleost fish remains unknown. Here, we generated a zebrafish scd mutant (scd-/-) to elucidate the role of scd in lipid metabolism and sexual development. Gas chromatography-mass spectrometry (GC-MS) showed that the scd-/-mutants had increased levels of saturated fatty acids C16:0 and C18:0, and decreased levels of monounsaturated fatty acids C16:1 and C18:1. The mutant fish displayed a short stature and an enlarged abdomen during development. Unlike Scd-/-mammals, the scd-/-zebrafish showed significantly increased fat accumulation in the whole body, especially in the liver, leading to hepatic mitochondrial dysfunction and severe cell apoptosis. Mechanistically, srebf1, a gene encoding a transcriptional activator related to adipogenesis, acc1 and acaca, genes involved in fatty acid synthesis, and dgat2, a key gene involved in triglyceride synthesis, were significantly upregulated in mutant livers to activate fatty acid biosynthesis and adipogenesis. The scd-/-males exhibited defective natural mating behavior due to defective genital papillae but possessed functional mature sperm. All defects in the scd-/-mutants could be rescued by ubiquitous transgenic overexpression of scd. In conclusion, our study demonstrates that scd is indispensable for maintaining lipid homeostasis and development of secondary sexual characteristics in zebrafish.
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