期刊
JOURNAL OF MARINE SCIENCE AND ENGINEERING
卷 10, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/jmse10122028
关键词
cosinor analysis; biological clock; circadian rhythm; gene expression
资金
- Guangxi Innovation Driven Development Special Fund Project [Guike AA18242031]
- Central Public-Interest Scientific Institution Basal Research Fund, CAFS [2020TD55]
- Central Public-Interest Scientific Institution Basal Research Fund South China Sea Fisheries Research Institute, CAFS [2021SD09]
This study demonstrates that the rhythm, lipid metabolism, and immune genes in the livers of mackerel tuna are influenced by time and weather, resulting in significant changes in gene expression.
In order to investigate the rhythmic changes in gene expression in the liver of mackerel tuna (Euthynnus affinis) under sunny and cloudy conditions, this experiment had four sampling times (6:00, 12:00, 18:00 and 24:00) set on sunny and cloudy days to determine the expression of their immune, metabolic and rhythmic genes. The results showed that daily rhythmicity was present within most of the rhythm genes (CREB1, CLOCK, PER1, PER2, PER3, REVERBA, CRY2 and BMAL1), metabolic genes (SIRT1 and SREBP1) and immune genes (NF-kB1, MHC-I, ALT, IFNA3, ISY1, ARHGEF13, GCLM and GCLC) in this study under the sunny and cloudy condition (p < 0.05). The expression levels of CREB1, PER1, PER3, RORA, REVERBA, CRY1 and BMAL1 within rhythm genes were significantly different (p < 0.05) in the same time point comparison between sunny and cloudy conditions at 6:00, 12:00, 18:00 and 24:00; metabolic genes had the expression levels of LPL at 6:00, 12:00, 18:00 and 24:00 in the same time point comparison (p < 0.05); immune genes only had significant differences in the expression levels of IFNA3 at 6:00, 12:00, 18:00 and 24:00 (p < 0.05). This study has shown that rhythm, lipid metabolism and immune genes in the livers of mackerel tuna are affected by time and weather and show significant changes in expression.
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