期刊
PLANTS-BASEL
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/plants12020252
关键词
abiotic stress; deletion analysis; natural rubber; phytohormones; promoter; small rubber particle protein
Taraxacum kok-saghyz is a model species for studying natural rubber biosynthesis due to its ability to produce high-quality rubber in its root. The stress-related gene small rubber particle protein (SRPP) is involved in natural rubber biosynthesis. This study investigated the transcriptional regulation of the TkSRPP promoter by analyzing different lengths of promoter derivatives fused with the beta-glucuronidase (GUS) reporter gene in tobacco. The results showed that the TkSRPP promoter was not tissue-specific and responded strongly to hormones and injury induction. This study provides valuable information for understanding the regulation of SRPP promoter and its role in natural rubber biosynthesis under hormonal and wounding stress.
Taraxacum kok-saghyz is a model species for studying natural rubber biosynthesis because its root can produce high-quality rubber. Small rubber particle protein (SRPP), a stress-related gene to multiple stress responses, involves in natural rubber biosynthesis. To investigate the transcriptional regulation of the TkSRPP promoter, the full-length promoter PR0 (2188 bp) and its four deletion derivatives, PR1 (1592 bp), PR2 (1274 bp), PR3 (934 bp), and PR4 (450 bp), were fused to beta-glucuronidase (GUS) reporter gene and transformed into tobacco. The GUS tissue staining showed that the five promoters distinctly regulated GUS expression utilizing transient transformation of tobacco. The GUS activity driven by a PR0 promoter was detected in transgenic tobacco leaves, stem and roots, suggesting that the TkSRPP promoter was not tissue-specific. Deletion analyses in transgenic tobacco have demonstrated that the PR3 from -934 bp to -450 bp core region responded strongly to the hormones, methyl jasmonate (MeJA), abscisic acid (ABA), and salicylic acid (SA), and also to injury induction. The TkSRPP gene was highly expressed under hormones and wound-induced conditions. This study reveals the regulation pattern of the SRPP promoter, and provides valuable information for studying natural rubber biosynthesis under hormones and wounding stress.
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