期刊
PLANTS-BASEL
卷 11, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/plants11243527
关键词
TPC1; vacuole; EF-domain; Physcomitrella; coordination of Ca2+
资金
- Agencia Nacional de Investigacion y Desarrollo de Chile (ANID ) [21220432, 21220419, 21190271]
- Anillo-ANID [ATE220043]
- National Institutes of Health (NIH) [R01-GM131043]
- Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica (FONDECYT) [3190544, 1210920, 1220504]
Through analyzing the genome of Physcomitrium patens, it was found that TPC1b channels have variations in critical amino acids, leading to a lower calcium ion coordination capacity compared to common TPC1 channels.
Two-pore channels (TPCs) are members of the superfamily of ligand-gated and voltage-sensitive ion channels in the membranes of intracellular organelles of eukaryotic cells. The evolution of ordinary plant TPC1 essentially followed a very conservative pattern, with no changes in the characteristic structural footprints of these channels, such as the cytosolic and luminal regions involved in Ca2+ sensing. In contrast, the genomes of mosses and liverworts encode also TPC1-like channels with larger variations at these sites (TPC1b channels). In the genome of the model plant Physcomitrium patens we identified nine non-redundant sequences belonging to the TPC1 channel family, two ordinary TPC1-type, and seven TPC1b-type channels. The latter show variations in critical amino acids in their EF-hands essential for Ca2+ sensing. To investigate the impact of these differences between TPC1 and TPC1b channels, we generated structural models of the EF-hands of PpTPC1 and PpTPC1b channels. These models were used in molecular dynamics simulations to determine the frequency with which calcium ions were present in a coordination site and also to estimate the average distance of the ions from the center of this site. Our analyses indicate that the EF-hand domains of PpTPC1b-type channels have a lower capacity to coordinate calcium ions compared with those of common TPC1-like channels.
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