Species- and Metal-Specific Responses of the Ionome of Three Duckweed Species under Chromate and Nickel Treatments

In this study, growth and ionomic responses of three duckweed species were analyzed, namely Lemna minor, Landoltia punctata, and Spirodela polyrhiza, were exposed for short-term periods to hexavalent chromium or nickel under laboratory conditions. It was found that different duckweed species had distinct ionomic patterns that can change considerably due to metal treatments. The results also show that, because of the stress-induced increase in leaf mass-to-area ratio, the studied species showed different order of metal uptake efficiency if plant area was used as unit of reference instead of the traditional dry weight-based approach. Furthermore, this study revealed that mu XRF is applicable in mapping elemental distributions in duckweed fronds. By using this method, we found that within-frond and within-colony compartmentation of metallic ions were strongly metal- and in part species-specific. Analysis of duckweed ionomics is a valuable approach in exploring factors that affect bioaccumulation of trace pollutants by these plants. Apart from remediating industrial effluents, this aspect will gain relevance in food and feed safety when duckweed biomass is produced for nutritional purposes.

Automated summary

In this study, the growth and ionomic responses of three species of duckweed, Lemna minor, Landoltia punctata, and Spirodela polyrhiza, to hexavalent chromium or nickel exposure were analyzed. The study found that different species of duckweed had distinct ionomic patterns that can vary under metal treatments. Additionally, the study showed that the order of metal uptake efficiency varied depending on whether plant area or dry weight was used as a reference unit. Furthermore, mu XRF was found to be an applicable method for mapping elemental distributions in duckweed fronds, revealing species-specific compartmentation of metallic ions.