Study on Black Spot Disease Detection and Pathogenic Process Visualization on Winter Jujubes Using Hyperspectral Imaging System

In this work, the potential of a hyperspectral imaging (HSI) system for the detection of black spot disease on winter jujubes infected by Alternaria alternata during postharvest storage was investigated. The HSI images were acquired using two systems in the visible and near-infrared (Vis-NIR, 400-1000 nm) and short-wave infrared (SWIR, 1000-2000 nm) spectral regions. Meanwhile, the change of physical (peel color, weight loss) and chemical parameters (soluble solids content, chlorophyll) and the microstructure of winter jujubes during the pathogenic process were measured. The results showed the spectral reflectance of jujubes in both the Vis-NIR and SWIR wavelength ranges presented an overall downtrend during the infection. Partial least squares discriminant models (PLS-DA) based on the HSI spectra in Vis-NIR and SWIR regions of jujubes both gave satisfactory discrimination accuracy for the disease detection, with classification rates of over 92.31% and 91.03%, respectively. Principal component analysis (PCA) was carried out on the HSI images of jujubes to visualize their infected areas during the pathogenic process. The first principal component of the HSI spectra in the Vis-NIR region could highlight the diseased areas of the infected jujubes. Consequently, Vis-NIR HSI and NIR HSI techniques had the potential to detect the black spot disease on winter jujubes during the postharvest storage, and the Vis-NIR HSI spectral information could visualize the diseased areas of jujubes during the pathogenic process.

Automated summary

This study investigated the potential of a hyperspectral imaging (HSI) system to detect black spot disease in winter jujubes infected by Alternaria alternata during postharvest storage. HSI images were obtained using two systems in the visible and near-infrared (Vis-NIR) and short-wave infrared (SWIR) spectral regions. Physical and chemical parameters, as well as the microstructure of winter jujubes, were measured during the pathogenic process. The results showed that the spectral reflectance of jujubes exhibited a decreasing trend in both the Vis-NIR and SWIR wavelength ranges. Partial least squares discriminant models based on the HSI spectra achieved satisfactory accuracy for disease detection.