Nutritional Quality, Techno-Functional Characteristics, and Safety of Biomass Powder and Protein Isolate Produced from Penicillium maximae

This study investigated the suitability of Penicillium maximae biomass powder and protein isolate as a food product or food ingredient. The biomass powder is rich in proteins (34.8%) and insoluble fiber (36.2%) but poor in lipids (3.1%). Strong water hydration (8.3 g/g, 8.5 g/g) and oil holding (6.9 g/g, 16.3 g/g) capacity were observed in the biomass powder and protein isolate, respectively, besides 100% emulsion stability, indicating multiple applications in the food industry. No locomotor impairment was induced in Drosophila melanogaster flies after consuming extracts of P. maximae biomass powder. Furthermore, decreased production of reactive oxygen species and preservation of survival, viability, and fertility parameters were observed in the nematode Caenorhabditis elegans, which reinforces the potential of P. maximae biomass for human and animal consumption. Together, the results show the vast food applicability of P. maximae biomass and protein isolate as protein substitutes with several health and environmental benefits.

Automated summary

This study explores the suitability of P. maximae biomass powder and protein isolate as food products or ingredients. The findings show that they consist of high levels of protein and insoluble fiber, with strong water hydration and oil holding capacities. The experiments conducted on fruit flies and nematodes demonstrate their potential positive effects on locomotor abilities, reactive oxygen species production, survival, viability, and fertility. These results highlight the wide range of food applications for P. maximae biomass and protein isolate as protein substitutes, offering numerous health and environmental benefits.