Protective Effect of [Cu(NN1)2](ClO4) Complex in Rainbow Trout Challenged against Flavobacterium psychrophilum

Previously, we reported an in vitro evaluation regarding antibacterial effects against F. psychrophilum by a new Cu (I) complex, [Cu(NN1)(2)](ClO4). This study presents the results of an in vivo evaluation of [Cu(NN1)(2)](ClO4) added as a dietary supplement against F. psychrophilum in rainbow trout. The results showed that the administration of [Cu(NN1)(2)](ClO4) at 29 and 58 mu g/g of fish for 15 days does not affect the growth of rainbow trout. On the other hand, the amount of copper present in the liver, intestine, and muscle of rainbow trout was determined. The results showed that the amount of copper in the liver, when compared between treated fish and control fish, does not change. While, in the intestine, an increase in the fish fed at 58 mu g/g of fish was observed. In muscle, a slight decrease at 29 mu g/g was obtained. Additionally, copper concentrations in the pond water after 15 days of feeding with the [Cu(NN1)(2)](ClO4) complex showed the highest levels of copper. Finally, the effect of the administration of [Cu(NN1)(2)](ClO4) for 15 days at 58 mu g/g of fish was evaluated against F. psychrophilum, where a 75% survival was obtained during 20 days of challenge.

Automated summary

The study showed that adding [Cu(NN1)(2)](ClO4) as a dietary supplement to rainbow trout for 15 days at doses of 29 and 58 μg/g fish did not affect growth, but resulted in changes in copper concentration in the intestine and muscle. High copper levels were detected in pond water after feeding with the complex. The administration of [Cu(NN1)(2)](ClO4) at 58 μg/g of fish for 15 days showed a 75% survival rate against F. psychrophilum.