期刊
ANTIOXIDANTS
卷 11, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/antiox11122330
关键词
SIRT1; resveratrol; proximity labeling; RanGap1; ROS
资金
- National Natural Science Foundation of China
- Innovation Capability Support Program of Shaanxi Province [31670781]
- [2018PT-28]
- [2017KTPT-04]
This study reveals the interaction between SIRT1 and RanGap1, and its influence on intracellular ROS levels. The findings highlight the importance of this interaction in mitochondrial functions, cell proliferation, and transcription.
SIRT1 functions by regulating the modification of proteins or interacting with other proteins to form complexes. It has been widely studied and found to play significant roles in various biological processes and diseases. However, systematic studies on activated-SIRT1 interactions remain limited. Here, we present a comprehensive SIRT1 interactome under resveratrol stimulation through proximity labeling methods. Our results demonstrated that RanGap1 interacted with SIRT1 in HEK 293T cells and MCF-7 cells. SIRT1 regulated the protein level of RanGap1 and had no obvious effect on RanGap1 transcription. Moreover, the overexpression of Rangap1 increased the ROS level in MCF-7 cells, which sensitized cells to resveratrol and reduced the cell viability. These findings provide evidence that RanGap1 interacts with SIRT1 and influences intracellular ROS, critical signals for mitochondrial functions, cell proliferation and transcription. Additionally, we identified that the SIRT1-RanGap1 interaction affects downstream signals induced by ROS. Overall, our study provides an essential resource for future studies on the interactions of resveratrol-activated SIRT1. There are conflicts about the relationship between resveratrol and ROS in previous reports. However, our data identified the impact of the resveratrol-SIRT1-RanGap1 axis on intracellular ROS.
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