期刊
ANTIOXIDANTS
卷 11, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/antiox11122448
关键词
Cedrela sinensis; chronic unpredictable mild stress; depression; mitochondrial function; inflammation; hormone changes
资金
- Basic Science Research Program through the National Research Foundation (NRF) of Republic of Korea
- Ministry of Education, Republic of Korea
- [NRF 2018R1D1A3B07043398]
This study evaluated the protective effects of ethyl acetate fraction from Cedrela sinensis (EFCS) against behavioral dysfunction and stress response induced by chronic unpredictable mild stress (CUMS) in mice. The results showed that EFCS alleviated depression-like behavior, inhibited mitochondrial dysfunction, regulated stress hormones, and downregulated inflammatory responses and apoptosis proteins in brain tissues.
This study aimed to evaluate the protective effects of ethyl acetate fraction from Cedrela sinensis (EFCS) against chronic unpredictable mild stress (CUMS)-induced behavioral dysfunction and stress response in C57BL/6 mice. The physiological compounds of EFCS were identified as rutin, isoquercitrin, ethyl gallate, quercitrin, kaempferol-3-O-rhamnoside, and ethyl digallate, using UPLC-Q-TOF/MSE. To evaluate the neuroprotective effect of EFCS, H2O2- and corticosterone-induced neuronal cell viability was conducted in human neuroblastoma MC-IXC cells. It was found that EFCS alleviated depression-like behavior by conducting the sucrose preference test (SPT), forced swimming test (FST), open field test (OFT), and tail suspension test (TST). EFCS inhibited mitochondrial dysfunction related to neuronal energy metabolism by regulating reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), and ATP contents in brain tissue. In addition, the administration of EFCS regulated the stress hormones in serum. EFCS regulated stress-related indicators such as CRF, ACTH, CYP11B1, and BDNF. Moreover, EFCS downregulated the inflammatory responses and apoptosis proteins such as caspase-1, TNF-alpha, IL-1 beta, p-JNK, BAX, and p-tau in brain tissues. These results suggest that EFCS might be a potential natural plant material that alleviates CUMS-induced behavior disorder by regulating inflammation in brain tissue against CUMS-induced depression.
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