期刊
BIOMOLECULES
卷 12, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/biom12121850
关键词
agronomic traits; chromosome segment substitution lines (CSSLs); common wild rice (Oryza rufipogon); molecular markers; QTL mapping; qLMC6; 1; qPH1
Wild rice is a valuable source for genes that can be used in breeding rice cultivars with beneficial traits. Chromosome segment substitution lines (CSSLs) provide a powerful tool for mapping quantitative trait loci (QTLs). In this study, 59 CSSLs were developed through backcrossing and marker-assisted selection (MAS) using common wild rice (Oryza rufipogon Griff.) as the donor and indica rice variety 93-11 as the recipient. Whole genome re-sequencing identified numerous molecular markers for these CSSLs, and through mapping analysis, QTLs related to economic traits were identified. The findings of this study have important implications for theoretical knowledge and genetic applications in rice breeding.
Wild rice is a primary source of genes that can be utilized to generate rice cultivars with advantageous traits. Chromosome segment substitution lines (CSSLs) are consisting of a set of consecutive and overlapping donor chromosome segments in a recipient's genetic background. CSSLs are an ideal genetic population for mapping quantitative traits loci (QTLs). In this study, 59 CSSLs from the common wild rice (Oryza rufipogon Griff.) accession DP15 under the indica rice cultivar (O. sativa L. ssp. indica) variety 93-11 background were constructed through multiple backcrosses and marker-assisted selection (MAS). Through high-throughput whole genome re-sequencing (WGRS) of parental lines, 12,565 mapped InDels were identified and designed for polymorphic molecular markers. The 59 CSSLs library covered 91.72% of the genome of common wild rice accession DP15. The DP15-CSSLs displayed variation in six economic traits including grain length (GL), grain width (GW), thousand-grain weight (TGW), grain length-width ratio (GLWR), plant height (PH), and leaf margin color (LMC), which were finally attributed to 22 QTLs. A homozygous CSSL line and a purple leave margin CSSL line were selected to construct two secondary genetic populations for the QTLs mapping. Thus, the PH-controlling QTL qPH1.1 was mapped to a region of 4.31-Mb on chromosome 1, and the LMC-controlling QTL qLMC6.1 was mapped to a region of 370-kb on chromosome 6. Taken together, these identified novel QTLs/genes from common wild rice can potentially promote theoretical knowledge and genetic applications to rice breeders worldwide.
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