期刊
BIOMOLECULES
卷 13, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/biom13010118
关键词
pulpitis; dental pulp stem cells; dentin; pulp regeneration; cannabidiol; inflammation
This study found that cannabidiol enhances the proliferation, migration, and osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). CBD also exhibits anti-inflammatory, chemotactic, anti-microbial, and tissue regenerative potentials. These findings suggest the potential application of CBD in dental pulp and bone regeneration based on DPSCs.
Strategies to promote dental pulp stem cells (DPSCs) functions including proliferation, migration, pro-angiogenic effects, and odontogenic/osteogenic differentiation are in urgent need to restore pulpitis-damaged dentin/pulp regeneration and DPSCs-based bone tissue engineering applications. Cannabidiol (CBD), an active component of Cannabis sativa has shown anti-inflammation, chemotactic, anti-microbial, and tissue regenerative potentials. Based on these facts, this study aimed to analyze the effect of CBD on DPSCs proliferation, migration, and osteogenic/odontogenic differentiation in basal and inflammatory conditions. Highly pure DPSCs with characteristics of mesenchymal stem cells (MSCs) were successfully isolated, as indicated by the results of flowcytometry and multi-lineage (osteogenic, adipogenic, and chondrogenic) differentiation potentials. Among the concentration tested (0.1-12.5 mu M), CBD (2.5 mu M) showed the highest anabolic effect on the proliferation and osteogenic/odontogenic differentiation of DPSCs. Pro-angiogenic growth factor VEGF mRNA expression was robustly higher in CBD-treated DPSCs. CBD also prompted the migration of DPSCs and CBD receptor CB1 and CB2 expression in DPSCs. TNF-alpha inhibited the viability, migration, and osteogenic/odontogenic differentiation of DPSCs and CBD reversed these effects. CBD alleviated the TNF-alpha-upregulated expression of pro-inflammatory cytokines TNF-alpha, interleukin (IL)-1 beta, and IL-6 in DPSCs. In conclusion, our results indicate the possible application of CBD on DPSCs-based dentin/pulp and bone regeneration.
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