期刊
JOURNAL OF RARE EARTHS
卷 34, 期 2, 页码 208-220出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1002-0721(16)60016-9
关键词
upconversion; luminescence; fluorescent dye; cell imaging; detection; rare earths
资金
- National Natural Science Foundation of China [21301058, 61376076, 61377024]
- Scientific Research Fund of Hunan Provincial Education Department [14B060]
- Natural Science Foundation of Hunan Province [13JJ4048]
Upconversion NaLuF4 nanoparticles were synthesized by the solvothermal method which could emit multicolor visible light under the excitation of 980 nm near-infrared (NIR) photons. These upconversion nanoparticles (UCNPs) with an acidic ligand could rapidly capture the basic rhodamine-B (RB) in plant cells to generate a close UCNPs@RB system. RB could efficiently absorb the green fluorescence from NaLuF4: 18 mol.%Yb3+, 2 mol.%Er3+ UCNPs and then emitted red light in the UCNPs@ RB system by a robust luminescence resonance energy transfer (LRET) from UCNPs to RB. The detection limit of RB with these upconversion fluorescent nanoprobes could reach 0.25 mu g/cm(3) in plant cell even under an ultra low excitation power source of 0.2 W/mm(2). This LRET phenomenon was also extended to NaLuF4: 18 mol.%Yb3+, 0.5 mol.%Tm3+@Sodium fluorescein (SF) system. In addition, the differential imaging could be achieved by successively incubating plant cells with fluorescent dyes and UCNPs. The fluorescent dyes aggregated in cell wall while UCNPs with surface modification distributed both in cell wall and cytoplasm, so that UCNPs@Dyes formed in cell walls which could emit multicolor light by LRET which was different from the emission in cytoplasm with only UCNPs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据