期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.1008436
关键词
cardiac tissue engineering; scaffolds; plasma treatment; surface functionalization; biocompatibility; polyvinylidene fluoride
资金
- LabEx REVIVE [ANR-10LABX-73]
- Ile-deFrance Region
- Fonds Marion Elizabeth Brancher
- AFM-Telethon [21833, 22142]
- DIM Respore
In this study, the hydrophilicity and biocompatibility of polyvinylidene fluoride (PVDF) scaffolds were enhanced using cold plasma treatment, showing potential application in cardiovascular tissue engineering.
As part of applications dealing with cardiovascular tissue engineering, drop-cast polyvinylidene fluoride (PVDF) scaffolds have been treated by cold plasma to enhance their adherence to cardiac cells. The scaffolds were treated in a dielectric barrier device where cold plasma was generated in a gaseous environment combining a carrier gas (helium or argon) with/without a reactive gas (molecular nitrogen). We show that an Ar-N-2 plasma treatment of 10 min results in significant hydrophilization of the scaffolds, with contact angles as low as 52.4 & DEG; instead of 132.2 & DEG; for native PVDF scaffolds. Correlation between optical emission spectroscopy and X-ray photoelectron spectroscopy shows that OH radicals from the plasma phase can functionalize the surface scaffolds, resulting in improved wettability. For all plasma-treated PVDF scaffolds, the adhesion and maturation of primary cardiomyocytes is increased, showing a well-organized sarcomeric structure (alpha-actinin immunostaining). The efficacy of plasma treatment was also supported by real-time PCR analysis to demonstrate an increased expression of the genes related to adhesion and cardiomyocyte function. Finally, the biocompatibility of the PVDF scaffolds was studied in a cardiac environment, after implantation of acellular scaffolds on the surface of the heart of healthy mice. Seven and 28 days after implantation, no exuberant fibrosis and no multinucleated giant cells were visible in the grafted area, hence demonstrating the absence of foreign body reaction and the biocompatibility of these scaffolds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据