4.7 Review

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2023.1020430

关键词

nucleic acid testing; rapid on-site detection; nucleic acid amplification; biosensor; microfluidic chip

向作者/读者索取更多资源

As nucleic acid testing becomes crucial in various research fields, there is an increasing demand for rapid on-site testing methods. This review discusses recent advances in on-chip methods for sample preparation, amplification, and detection in the nucleic acid testing procedure. It covers integration strategies for sample preparation, various on-chip amplification methods, and evaluation of potential detection methods. The review also highlights the importance of improving the speed and integration of all steps onto a single chip for future developments in nucleic acid testing.
As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection. This review covers recent advances in on-chip methods for each of these three steps and explains the principles underlying related methods. The sample preparation process is further divided into cell lysis and nucleic acid purification, and methods for the integration of these two steps on a single chip are discussed. Under amplification, on-chip studies based on PCR and isothermal amplification are covered. Three isothermal amplification methods reported to have good resistance to PCR inhibitors are selected for discussion due to their potential for use in direct amplification. Chip designs and novel strategies employed to achieve rapid extraction/amplification with satisfactory efficiency are discussed. Four detection methods providing rapid responses (fluorescent, optical, and electrochemical detection methods, plus lateral flow assay) are evaluated for their potential in rapid on-site detection. In the final section, we discuss strategies to improve the speed of the entire procedure and to integrate all three steps onto a single chip; we also comment on recent advances, and on obstacles to reducing the cost of chip manufacture and achieving mass production. We conclude that future trends will focus on effective nucleic acid extraction via combined methods and direct amplification via isothermal methods.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据