期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2022.983712
关键词
reproductive cycle; estrous cycle; systems biology; RNA-sequencing; hypertension
资金
- Peking University Basic Research Program
- PKU-Baidu Fund
- National Key RD Program
- National Natural Science Foundation of China
- [BMU2020JC001]
- [2019BD014]
- [2020YFC2004704]
- [81670462/81970440/81921001/81870551/82025008/82070833/81670777]
This study provides a comprehensive transcriptomic landscape of female mice during different phases of the reproductive cycle, revealing the widespread regulation of neuro-functions, immune functions, and blood coagulation. The study also identifies key transcription factors and pathways involved in the estrous cycle. Moreover, the study demonstrates the significant impact of the estrous cycle on the cardiovascular system and highlights the enhanced efficacy of certain hypotensive drugs during the estrus phase. Overall, this research contributes valuable insights for investigating the reproductive cycle and precision medicine.
Female reproductive cycle, also known as menstrual cycle or estrous cycle in primate or non-primate mammals, respectively, dominates the reproductive processes in non-pregnant state. However, in addition to reproductive tissues, reproductive cycle could also perform global regulation because the receptors of two major female hormones fluctuating throughout the cycle, estrogen and progesterone, are widely distributed. Therefore, a multi-tissue gene expression landscape is in continuous demand for better understanding the systemic changes during the reproductive cycle but remains largely undefined. Here we delineated a transcriptomic landscape covering 15 tissues of C57BL/6J female mice in two phases of estrous cycle, estrus and diestrus, by RNA-sequencing. Then, a number of genes, pathways, and transcription factors involved in the estrous cycle were revealed. We found the estrous cycle could widely regulate the neuro-functions, immuno-functions, blood coagulation and so on. And behind the transcriptomic alteration between estrus and diestrus, 13 transcription factors may play important roles. Next, bioinformatics modeling with 1,263 manually curated gene signatures of various physiological and pathophysiological states systematically characterized the beneficial/deleterious effects brought by estrus/diestrus on individual tissues. We revealed that the estrous cycle has a significant effect on cardiovascular system (aorta, heart, vein), in which the anti-hypertensive pattern in aorta induced by estrus is one of the most striking findings. Inspired by this point, we validated that two hypotensive drugs, felodipine and acebutolol, could exhibit significantly enhanced efficacy in estrus than diestrus by mouse and rat experiments. Together, this study provides a valuable data resource for investigating reproductive cycle from a transcriptomic perspective, and presents models and clues for investigating precision medicine associated with reproductive cycle.
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