Conditioned medium from human cord blood mesenchymal stem cells attenuates age-related immune dysfunctions

Aging is accompanied with progressive deterioration of immune responses and tissue's function. Using 12-month-old mice as model, we showed that conditioned medium of human cord blood mesenchymal stem cells (CBMSC-CM) significantly reduced the population percentage of CD3(-)CD335(+) NK and CD4(+)CD25(+) regulatory T-cells in peripheral blood. The CBMSC-CM administration also increased naive T-cells number and restored the ratio of naive to memory T-cells in CD4(+) T-cells population. These results indicated that CBMSC-CM improved the immune response efficiency of aged mice. Moreover, we also found CBMSC-CM treatment significantly reduced the number of senescenT-cells in kidney tissues. Finally, we demonstrated that CBMSC-CM remarkably attenuated hydrogen peroxide triggered T-cell response and ameliorated oxidative stress induced cellular senescence. All of these data suggest a prominent anti-aging effect of secretome of CBMSCs.

Automated summary

In this study, we demonstrated that conditioning medium derived from human cord blood mesenchymal stem cells (CBMSC-CM) improved immune response efficiency and reduced cellular senescence in aged mice. CBMSC-CM administration resulted in a decrease in NK and regulatory T-cell populations in peripheral blood, an increase in naive T-cell number, and restoration of the ratio of naive to memory T-cells in CD4(+) T-cell population. Additionally, CBMSC-CM treatment reduced the number of senescent T-cells in kidney tissues and attenuated hydrogen peroxide triggered T-cell response and oxidative stress induced cellular senescence.