期刊
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
卷 36, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.teac.2022.e00184
关键词
ssDNA Aptamers; Toxic chemical contaminants; Biosensing; Detection; Quantification; Food safety; Human Health; Water analysis; Environment
资金
- BioGreen21 Agri-Tech Innovation Program, Rural Development Administration, Republic of Korea [PJ01623501]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Republic of Korea [2020R1A6A1A03044344, 2020R1F1A1074027, 2022R1I1A1A01064372]
- National Research Foundation of Korea [2020R1F1A1074027, 2022R1I1A1A01064372] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This article provides an update on the advances of nucleic acid-based aptameric sensors for molecular diagnostics of toxic chemicals from food, water, human fluids, and the environment.
Detection of toxic small molecule contaminants with sensitivity, accuracy, and specificity is a challenging task. Traditionally used HPLC and mass spectrometry-based assays suffer from several drawbacks, including lengthy sample preparation, heavy instrumentation, and the need for expert technicians. Specific, measurable, accurate, robust, and time-saving (SMART) biosensors are needed to detect toxic substances. Aptamers provide unique opportunities for the rapid development of SMART biosensors to meet above challenges. Since aptamers are short nucleotide sequences; they are easy for chemical synthesis and functional modifications. Aptamers acquire specific molecule recognition potential through unique chemical bonding, including H-bonds, pi-pi, van der Waals, and hydrophobic interactions. For the discovery of aptamers, the SELEX process is used. Recently, efforts have been made to develop aptamers to detect toxic small molecules like antibiotics, pesticides, insecticides, pollutants, toxins, and allergens. Aptamer technology is a promising tool for analyzing these chemicals from diverse matrices. This review provides an update on advances in nucleic acid-based aptameric sensors for mo-lecular diagnostics of toxic chemical from food, water, human fluids, and the environment.
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