Toxicity and Preventive Activity of Chitosan, Equisetum arvense, Lecithin and Salix Cortex against Plasmopara viticola, the Causal Agent of Downy Mildew in Grapevine

Grapevine, a crop of global economic importance, is annually affected by diseases that can compromise the quality and quantity of the harvest, producing large economic losses. Downy mildew caused by Plasmopara viticola (Berk. & M.A. Curtis) Berl. & de Toni is one of the most important diseases in the vineyard. To fight this pathogen, winegrowers often rely on conventional chemical fungicides or copper-based formulations, whose use is determined to be reduced by the European Commission due to their environmental consequences. Hence, alternative plant protection products (PPP) in grapevine must be considered and studied. In this context, we selected several alternative commercial products, based on basic substances (BS) or low-risk active substances (LRAS), to evaluate their suitability to deal with P. viticola. We measured the preventive activity of the products, both in vitro and in planta, as well as their toxicity against the sporangia and zoospores of the pathogen. Results showed that four commercial products were effective against the pathogen directly and preventively, being composed of approved basic substances, more concretely, chitosan, Equisetum arvense, lecithins, and Salix cortex. Among those, the products composed of lecithins and Salix cortex were the most toxic and active preventively. Therefore, these basic substances should be promoted in the vineyard as an alternative to conventional treatments in order to transition to a more sustainable viticulture.

Automated summary

Grapevine is annually affected by diseases, such as downy mildew caused by Plasmopara viticola, which can lead to economic losses. Conventional chemical fungicides and copper-based formulations used to combat these diseases have environmental consequences. Therefore, alternative plant protection products (PPP) containing basic substances like chitosan, Equisetum arvense, lecithins, and Salix cortex can be promoted as more sustainable treatments for grapevine diseases.