期刊
AGRONOMY-BASEL
卷 12, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/agronomy12122946
关键词
COP9 signalosome; CSN1; rice; SLR1
The COP9 signalosome (CSN), particularly CSN1, plays a crucial role in plant development. This study focused on investigating the role of CSN1 in rice using the CRISPR/Cas9 system. The results showed that OsCSN1 acts as a negative regulator in seedling growth and development by promoting the degradation of the SLR1 protein in the GA signaling pathway.
The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) is involved in various regulations during plant development. The CSN is a highly conserved protein complex with nine subunits, and CSN1 acts in a network of signaling pathways critical for plant development. Although CSN1 has been widely studied in Arabidopsis thaliana, there have been few investigations on CSN1 in rice. In this paper, using the CRISPR/Cas9 system, CSN1 was edited from Oryza sativa subsp. japonica (rice). After screening out the OsCSN1 knockout mutant and OsCSN1 reduce mutant, the phenotype and protein expression were identified under different light conditions. Experiments showed that in OsCSN1 knockout mutant and OsCSN1 reduce mutant, the SLR1 protein was rapidly degraded at the rice seedling. In this study, the OsCSN1 acted as a negative regulator to affect seedling growth and development through CUL4-based E3 ligase, which is involved in the degradation of SLR1 in the GA signaling pathway. However, its direct target and mechanism of action are not clear.
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