Biologic Impact of Green Synthetized Magnetic Iron Oxide Nanoparticles on Two Different Lung Tumorigenic Monolayers and a 3D Normal Bronchial Model-EpiAirway (TM) Microtissue

The present study reports the successful synthesis of biocompatible magnetic iron oxide nanoparticles (MNPs) by an ecofriendly single step method, using two ethanolic extracts based on leaves of Camellia sinensis L. and Ocimum basilicum L. The effect of both green raw materials as reducing and capping agents was taken into account for the development of MNPs, as well as the reaction synthesis temperature (25 degrees C and 80 degrees C). The biological effect of the MNPs obtained from Camellia sinensis L. ethanolic extract (Cs 25, Cs 80) was compared with that of the MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80), by using two morphologically different lung cancer cell lines (A549 and NCI-H460); the results showed that the higher cell viability impairment was manifested by A549 cells after exposure to MNPs obtained from Ocimum basilicum L. ethanolic extract (Ob 25, Ob 80). Regarding the biosafety profile of the MNPs, it was shown that the EpiAirway (TM) models did not elicit important viability decrease or significant histopathological changes after treatment with none of the MNPs (Cs 25, Cs 80 and Ob 25, Ob 80), at concentrations up to 500 mu g/mL.

Automated summary

The study successfully synthesized biocompatible magnetic iron oxide nanoparticles (MNPs) using ethanolic extracts from Camellia sinensis L. and Ocimum basilicum L. leaves. The MNPs obtained from Ocimum basilicum L. extract showed higher cell viability impairment on lung cancer cell lines (A549) compared to those obtained from Camellia sinensis L. extract. The biosafety evaluation using EpiAirwayTM models showed no significant decrease in viability or histopathological changes after treatment with the MNPs.