Selective Induction of Intrinsic Apoptosis in Retinoblastoma Cells by Novel Cationic Antimicrobial Dodecapeptides

Host defense peptides represent an important component of innate immunity. In this work, we report the anticancer properties of a panel of hyper-charged wholly cationic antimicrobial dodecapeptides (CAPs) containing multiple canonical forms of lysine and arginine residues. These CAPs displayed excellent bactericidal activities against a broad range of pathogenic bacteria by dissipating the cytoplasmic membrane potential. Specifically, we identified two CAPs, named HC3 and HC5, that effectively killed a significant number of retinoblastoma (WERI-Rb1) cells (p <= 0.01). These two CAPs caused the shrinkage of WERI-Rb1 tumor spheroids (p <= 0.01), induced intrinsic apoptosis in WERI-Rb1 cells via activation of caspase 9 and caspase 3, cleaved the PARP protein, and triggered off the phosphorylation of p53 and gamma H2A.X. Combining HC3 or HC5 with the standard chemotherapeutic drug topotecan showed synergistic anti-cancer activities. Overall, these results suggest that HC3 and HC5 can be exploited as potential therapeutic agents in retinoblastoma as monotherapy or as adjunctive therapy to enhance the effectiveness of currently used treatment modalities.

Automated summary

Host defense peptides, namely hyper-charged wholly cationic antimicrobial dodecapeptides (CAPs), demonstrated excellent bactericidal activities against pathogenic bacteria and exhibited potent anticancer properties against retinoblastoma cells. Two CAPs, HC3 and HC5, were found to induce apoptosis in retinoblastoma cells and showed synergistic effects when combined with the chemotherapeutic drug topotecan.