期刊
FRONTIERS IN ONCOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2022.1063477
关键词
adenoid cystic carcinoma; head and neck cancer; single-cell transcriptomic analysis; MYB; EN1
类别
资金
- National Key Research and Development Program [2017YFB1304300, 2020YFC2003405]
- National Natural Science Foundation of China [81800939]
- Youth Incubation Program of Medical Science and Technology of PLA [21QNPY114]
In this study, we used single-cell RNA sequencing to analyze the transcriptomes of cells from paracarcinoma and carcinoma tissues of ACC patients. We identified different types of epithelial cells and found that specific intercalated duct-like cells with copy number variations were premalignant cells that eventually transformed into malignant cells. Additionally, we discovered that MYB and MYBL1 belong to different gene modules and drive ACC progression in different directions.
Adenoid cystic carcinoma (ACC) is a malignant tumor that originates from exocrine gland epithelial cells. We profiled the transcriptomes of 49,948 cells from paracarcinoma and carcinoma tissues of three patients using single-cell RNA sequencing. Three main types of the epithelial cells were identified into myoepithelial-like cells, intercalated duct-like cells, and duct-like cells by marker genes. And part of intercalated duct-like cells with special copy number variations which altered with MYB family gene and EN1 transcriptomes were identified as premalignant cells. Developmental pseudo-time analysis showed that the premalignant cells eventually transformed into malignant cells. Furthermore, MYB and MYBL1 were found to belong to two different gene modules and were expressed in a mutually exclusive manner. The two gene modules drove ACC progression into different directions. Our findings provide novel evidence to explain the high recurrence rate of ACC and its characteristic biological behavior.
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