A Promising Ash Supplementation Strategy in the Cultivation of Spirodela polyrrhiza Plants

An innovative approach to the management of waste in the form of ash obtained during biomass combustion is justified due to its specific properties, including the presence of macro- and microelements. The aim of the current study was to determine the concentration of ash obtained from Sorghum combustion regarding its fertilizer value and its effect on the cytological structures, physiological parameters, growth and development of Lemnaceae plants, thereby demonstrating the possibility of using this waste to supplement culture media. The analyses showed that the use of ash in the in vitro cultivation of Lemnaceae aquatic plants had a dose-dependent effect. The addition of 2% ash favorably affected the condition of plant roots, i.e., meristem elongation and an increase in nucleoli sizes as well as improving the chlorophyll content index, gas exchange parameters, chemical oxygen demand (COD) and plant vigor via PSII, which was confirmed by a chlorophyll fluorescence measurement. On the other hand, too high of a concentration, i.e., 10% ash, adversely affected the plant development and parameters studied. Concluding, the use of ash at a low concentration favorably affected the yielding of Spirodela polyrrhiza, whose biomass can be used for energy purposes in the production of bioethanol, plant biogas or the phytoremediation of industrial waters and leachate.

Automated summary

An innovative method of managing ash waste from biomass combustion was studied. The research aimed to determine the fertilizer value of ash from Sorghum combustion and its effects on Lemnaceae plants. The use of ash in the in vitro cultivation of Lemnaceae plants showed a dose-dependent effect, with 2% ash improving various physiological parameters. However, a high concentration of 10% ash adversely affected plant development. The use of low concentrations of ash positively influenced the yielding of Spirodela polyrrhiza.