期刊
CELLS
卷 11, 期 23, 页码 -出版社
MDPI
DOI: 10.3390/cells11233928
关键词
CRISPR; Cas9; Cas12; Cas13; base editing; Prime Editing; biotic and abiotic stresses
类别
资金
- National Key Research and Development Program of China
- National Natural Science Foundation of China
- Special projects for Foreign Cooperation of Yunnan Province
- Hubei Hongshan Laboratory
- Fundamental Research Funds for the Central Universities
- [2021YFD1400800]
- [31872077]
- [202003AD150014]
- [2021hszd013]
- [2021ZKPY003]
CRISPR/Cas-mediated genome editing is a game-changing tool for rapidly improving desirable traits in crops. It allows for easy applications, high efficiency, and low cost in producing targeted edits, leading to increased quality and yield in a shorter time. This technology holds great potential for creating crop varieties with resistance to biotic and abiotic stresses.
Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game-changing tool that can create variation in desired traits, such as biotic and abiotic resistance, increase quality and yield in less time with easy applications, high efficiency, and low cost in producing the targeted edits for rapid improvement of crop plants. Plant pathogens and the severe environment cause considerable crop losses worldwide. GE approaches have emerged and opened new doors for breeding multiple-resistance crop varieties. Here, we have summarized recent advances in CRISPR/Cas-mediated GE for resistance against biotic and abiotic stresses in a crop molecular breeding program that includes the modification and improvement of genes response to biotic stresses induced by fungus, virus, and bacterial pathogens. We also discussed in depth the application of CRISPR/Cas for abiotic stresses (herbicide, drought, heat, and cold) in plants. In addition, we discussed the limitations and future challenges faced by breeders using GE tools for crop improvement and suggested directions for future improvements in GE for agricultural applications, providing novel ideas to create super cultivars with broad resistance to biotic and abiotic stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据