期刊
CELLS
卷 11, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/cells11244066
关键词
alopecia; dermal papilla; extracellular vesicles; engineered nanovesicles
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education
- [NRF-2019R1I1A1A01061296]
- [NRF-2021R1I1A1A01040732]
- [NRF-2022R1I1A1A01068652]
The study suggests that engineered nanovesicles (eNVs) have therapeutic potential for alopecia, as they can enhance the proliferation and migration of hair follicle cells and increase the size of human hair follicles.
Alopecia is a common medical condition affecting both sexes. Dermal papilla (DP) cells are the primary source of hair regeneration in alopecia patients. Therapeutic applications of extracellular vesicles (EVs) are restricted by low yields, high costs, and their time-consuming collection process. Thus, engineered nanovesicles (eNVs) have emerged as suitable therapeutic biomaterials in translational medicine. We isolated eNVs by the serial extrusion of fibroblasts (FBs) using polycarbonate membrane filters and serial and ultracentrifugation. We studied the internalization, proliferation, and migration of human DP cells in the presence and absence of FB-eNVs. The therapeutic potential of FB-eNVs was studied on ex vivo organ cultures of human hair follicles (HFs) from three human participants. FB-eNVs (2.5, 5, 7.5, and 10 mu g/mL) significantly enhanced DP cell proliferation, with the maximum effect observed at 7.5 mu g/mL. FB-eNVs (5 and 10 mu g/mL) significantly enhanced the migration of DP cells at 36 h. Western blotting results suggested that FB-eNVs contain vascular endothelial growth factor (VEGF)-a. FB-eNV treatment increased the levels of PCNA, pAKT, pERK, and VEGF-receptor-2 (VEGFR2) in DP cells. Moreover, FB-eNVs increased the human HF shaft size in a short duration ex vivo. Altogether, FB-eNVs are promising therapeutic candidates for alopecia.
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