期刊
CELLS
卷 11, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/cells11213495
关键词
platelets; Escherichia coli; platelet clumps; platelet activation; platelet aggregation; microscopy
类别
资金
- InstitutHospitalo-Universitaire (IHU) MediterraneeInfection, Marseille, France
- Algerian Ministry of Higher Education and Scientific Research
The study investigated the structure of platelet aggregates induced by different E. coli strains and the ultrastructure of platelet-E. coli mixtures using SEM and TEM. The results showed that the appearance of platelet aggregates was mainly dependent on the strain used, indicating a potential relationship between the structure of E. coli strains and platelet activation.
In addition to their role in haemostasis, platelets are also involved in the inflammatory and antimicrobial process. Interactions between pathogens and platelets, mediated by receptors can lead to platelet activation, which may be responsible for a granular secretion process or even aggregation, depending on the bacterial species. Granular secretion releases peptides with bactericidal activity as well as aggregating factors. To our knowledge, these interactions have been poorly studied for Escherichia coli (E. coli). Few studies have characterised the cellular organization of platelet-E. coli aggregates. The objective of our study was to investigate the structure of platelet aggregates induced by different E. coli strains as well as the ultrastructure of platelet-E. coli mixtures using a scanning and transmission electron microscopy (SEM and TEM) approach. Our results show that the appearance of platelet aggregates is mainly dependent on the strain used. SEM images illustrate the platelet activation and aggregation and their colocalisation with bacteria. Some E. coli strains induce platelet activation and aggregation, and the bacteria are trapped in the platelet magma. However, some strains do not induce significant platelet activation and are found in close proximity to the platelets. The structure of the E. coli strains might explain the results obtained.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据