期刊
SCIENCE ADVANCES
卷 8, 期 51, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.add3479
关键词
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资金
- Biotechnology and Biological Sciences Research Council [BB/ N007816/1]
- Medical Research Council [MR/P007503/1]
Gene transcription requires DNA melting and opening to form transcription initiation complexes. In bacteria, this process is mediated by RNA polymerase (RNAP) and sigma factors. A study found that a specific sigma factor (sigma(54)) interacts with an AAA+ activator to facilitate DNA melting and opening.
Gene transcription is carried out by RNA polymerase (RNAP) and requires the conversion of the initial closed promoter complex, where DNA is double stranded, to a transcription-competent open promoter complex, where DNA is opened up. In bacteria, RNAP relies on sigma factors for its promoter specificities. Using a special form of sigma factor (sigma(54)), which forms a stable closed complex and requires its activator that belongs to the AAA+ ATPases (ATPases associated with diverse cellular activities), we obtained cryo-electron microscopy structures of transcription initiation complexes that reveal a previously unidentified process of DNA melting opening. The sigma(54) amino terminus threads through the locally opened up DNA and then becomes enclosed by the AAA+ hexameric ring in the activator-bound intermediate complex. Our structures suggest how ATP hydrolysis by the AAA+ activator could remove the sigma(54) inhibition while helping to open up DNA, using sigma(54) amino-terminal peptide as a pry bar.
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