4.8 Article

Conserved meiotic mechanisms in the cnidarian Clytia hemisphaerica revealed by Spo11 knockout

期刊

SCIENCE ADVANCES
卷 9, 期 4, 页码 -

出版社

AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.add2873

关键词

-

向作者/读者索取更多资源

During meiosis, DNA recombination mediated by Spo11 enzyme triggers double-strand breaks (DSBs), which lead to the shuffling of genetic information between maternal and paternal chromosomes. In a study on the jellyfish Clytia hemisphaerica, they found that knocking down the Spo11 gene resulted in the failure of germ cells to assemble synaptonemal complexes and chiasmata, leading to the presence of unassociated homologous chromosome pairs and aneuploid but fertilizable eggs. This study provides significant evolutionary perspectives on meiosis regulation, as Clytia belongs to the sister clade of classical animal model species.
During meiosis, DNA recombination allows the shuffling of genetic information between the maternal and pa-ternal chromosomes. Recombination is initiated by double-strand breaks (DSBs) catalyzed by the conserved enzyme Spo11. How this crucial event is connected to other meiotic processes is unexpectedly variable depend-ing on the species. Here, we knocked down Spo11 by CRISPR in the jellyfish Clytia hemisphaerica. Germ cells in Clytia Spo11 mutants fail to assemble synaptonemal complexes and chiasmata, and in consequence, homolo-gous chromosome pairs in females remain unassociated during oocyte growth and meiotic divisions, creating aneuploid but fertilizable eggs that develop into viable larvae. Clytia thus shares an ancient eukaryotic depen-dence of synapsis and chromosome segregation on Spo11-generated DSBs. Phylogenetically, Clytia belongs to Cnidaria, the sister clade to Bilateria where classical animal model species are found, so these results provide fresh evolutionary perspectives on meiosis regulation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据