期刊
SCIENCE ADVANCES
卷 8, 期 51, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.ade5155
关键词
-
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) [18H05494, 21H00393, 20H04669]
- Japan Society for the Promotion of Science (JSPS) [19H03013, 15 K07383, 21H01772]
- Environment Research and Technology Development Fund from the Environmental Restoration and Conservation Agency of Japan (ERCA) [JPMEERF21S11900, JPMEERF21S11902]
- Business Finland
- U.S. Department of Energy (DOE) [DE-AC36-08GO28308]
- U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office
- Basic Energy Science office within the DOE Office of Science [DE-FG02-91ER20021]
- Department of Energy's Office of Energy Efficiency and Renewable Energy located at the National Renewable Energy Laboratory
Efficient depolymerization of crystalline cellulose requires the cooperative action of multiple cellulolytic enzymes. This study demonstrates that the synergistic activity between cellobiohy-drolases and a lytic polysaccharide monooxygenase can enhance the activity and performance of the enzymes by producing chain breaks and amorphizing the crystalline surface of cellulose.
Efficient depolymerization of crystalline cellulose requires cooperation between multiple cellulolytic enzymes. Through biochemical approaches, molecular dynamics (MD) simulation, and single-molecule observations using high-speed atomic force microscopy (HS-AFM), we quantify and track synergistic activity for cellobiohy-drolases (CBHs) with a lytic polysaccharide monooxygenase (LPMO) from Phanerochaete chrysosporium. Increas-ing concentrations of LPMO (AA9D) increased the activity of a glycoside hydrolase family 6 CBH, Cel6A, whereas the activity of a family 7 CBH (Cel7D) was enhanced only at lower concentrations of AA9D. MD simulation sug-gests that the result of AA9D action to produce chain breaks in crystalline cellulose can oxidatively disturb the crystalline surface by disrupting hydrogen bonds. HS-AFM observations showed that AA9D increased the number of Cel7D molecules moving on the substrate surface and increased the processivity of Cel7D, thereby increasing the depolymerization performance, suggesting that AA9D not only generates chain ends but also amorphizes the crystalline surface, thereby increasing the activity of CBHs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据