One-Pot Synthesis of Doxorubicin-Bioactive Glass-Ceramic Hybrid Nanoparticles through a Bio-Inspired Route for Anti-Cancer Therapy

In this study, we report doxorubicin preparation with bioactive glass-ceramic as a novel hybrid nanoparticle formulation. Synthesis was carried out through a bio-inspired route where different precursors of bioactive glass are added to a Tris(hydroxymethyl)aminomethane buffer adjusted to slightly alkaline pH containing doxorubicin. With increasing concentration of doxorubicin, loading increased in quantity with comparatively higher drug release in acidic pH than neutral pH. Without synthetic surfactant or high temperature calcination, bioactive glass-ceramic nanoparticles demonstrated significantly superior cytotoxic behavior towards osteosarcoma cell line when compared to equivalent free drug or its action towards non-cancerous cell line. The particles exhibited hydroxyapatite deposition when immersed in simulated body fluid for 7 days. The bio-inspired route for synthesis of doxorubicin-bioactive glass-ceramic hybrid nanoparticles is an efficient cost -effective synthesis mechanismwhich is environmentally friendly without hampering the stability or activity of the drug.

Automated summary

In this study, a novel hybrid nanoparticle formulation of doxorubicin with bioactive glass-ceramic was prepared. The synthesis was carried out through a bio-inspired route using different precursors of bioactive glass added to a Tris(hydroxymethyl)aminomethane buffer with slightly alkaline pH. The doxorubicin-bioactive glass-ceramic nanoparticles showed superior cytotoxic behavior towards osteosarcoma cells compared to free drug, without the use of surfactant or high temperature. The nanoparticles also exhibited hydroxyapatite deposition when immersed in simulated body fluid.