期刊
FOOD BIOSCIENCE
卷 50, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2022.102186
关键词
GPCR; Taste receptor; Floridoside; Pyropia yezoensis
资金
- JSPS KAKENHI, Japan [20K05927]
This study investigates the interaction between Isofloridoside (IF) and the sweet taste receptor T1R2/T1R3, showing that IF interacts with T1R2/T1R3 and has the potential as a new alternative sweetener.
Isofloridoside (IF) is a naturally occurring galactosylglycerol compound with a refreshing sweet taste. It is found in red algae, where it acts as a photosynthetic intermediate. However, the mechanism by which IF elicits sweetness has not been determined. In this study, we investigate the interaction between IF and the sweet taste receptor T1R2/T1R3. We show that IF causes an increase in intracellular Ca2+ concentration and an increase in Erk phosphorylation in T1R2/T1R3-expressing HEK293T cells, indicating that IF interacts with T1R2/T1R3. IF also activates endogenously expressed T1R2/T1R3 in mouse small intestinal endocrine L-cells, and promotes the secretion of the incretin glucagon-like peptide-1 (GLP-1). In silico docking simulations of IF and T1R2/T1R3 predict that IF forms hydrogen bonds with Tyr103 in the extracellular Venus flytrap domain of T1R2. This result is consistent with the binding mode of many other sweet-tasting molecules. We found that IF have the potential as a new alternative sweetener.
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