期刊
JOURNAL OF PROTEOMICS
卷 142, 期 -, 页码 70-81出版社
ELSEVIER
DOI: 10.1016/j.jprot.2016.05.009
关键词
Porcine reproductive and respiratory syndrome virus (PRRSV); Nonstructural protein 2 (nsp2); Interactome; Stable isotope labeling with amino acids in cell culture (SILAC); Immunoprecipitation
资金
- National Natural Science Foundation of China [31490602, 31225027, 31372467]
- National Basic Research Program (973) of China [2014CB542700]
- Research Fund for the Doctoral Program of Higher Education of China [20130146110001]
- Natural Science Foundation of Hubei Province [2014CFA009]
Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has heavily impacted the global swine industry. The PRRSV nonstructural protein 2 (nsp2) plays crucial roles in viral replication and host immune regulation, most likely by interacting with viral or cellular proteins that have not yet been identified. In this study, a quantitative interactome approach based on immunoprecipitation and stable isotope labeling with amino acids in cell culture (SILAC) was performed to identify nsp2-interacting proteins in PRRSV-infected cells with an nsp2-specific monoclonal antibody. Nine viral proteins and 62 cellular proteins were identified as potential nsp2-interacting partners. Our data demonstrate that the PRRSV nsp1 alpha, nsp1 beta, and nucleocapsid proteins all interact directly with nsp2. Nsp2-interacting cellular proteins were classified into different functional groups and an interactome network of nsp2 was generated. Interestingly, cellular vimentin, a known receptor for PRRSV, forms a complex with nsp2 by using viral nucleocapsid protein as an intermediate. Taken together, the nsp2 interactome under the condition of virus infection clarifies a role of nsp2 in PRRSV replication and immune evasion. Biological significance: Viral proteins must interact with other virus-encoded proteins and/or host cellular proteins to function, and interactome analysis is an ideal approach for identifying such interacting proteins. In this study, we used the quantitative interactome methodology to identify the viral and cellular proteins that potentially interact with the nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) under virus infection conditions, thus providing a rich source of potential viral and cellular interaction partners for PRRSV nsp2. Based on the interactome data, we further demonstrated that PRRSV nsp2 and nucleocapsid protein together with cellular vimentin, form a complex that may be essential for viral attachment and replication, which partly explains the role of nsp2 in PRRSV replication and immune evasion. (C) 2016 Elsevier B.V. All rights reserved.
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