Pomegranate attenuates kidney injury in cyclosporine-induced nephrotoxicity in rats by suppressing oxidative stress

To investigate the effect of pomegranate juice (PJ) on the cyclosporine (CsA)-induced nephrotoxicity in rats, 80 rats were divided into four groups. The first group was regarded a negative control group, and the others were as follows: group 2 (CsA group) received CsA in a dose of 25 mg/kg/day orally, group 3 (treated group) received CsA in a dose of 25 mg/kg/day plus 2.5 mL/day of PJ, and group 4 (PJ group) received 2.5 mL of PJ daily. By the end of the 21st day, plasma creatinine, blood urea nitrogen (BUN), creatinine clearance, urinary KIM-1, and NGAL were determined. Histopathological investigation and the determination of malondialdehyde and antioxidant enzymes were analyzed in kidney tissues. The results show that plasma creatinine, BUN, creatinine clearance, and kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin were significantly altered in the CsA group. The supplement of PJ attenuated the alteration in these parameters. The treatment with PJ also prohibits the CsA-induced alteration in the histopathology, lipid peroxidation, and antioxidant enzymes. We can conclude that PJ protects against CsA-induced nephrotoxicity due to its antioxidant effects.

Automated summary

This study aimed to investigate the effect of pomegranate juice (PJ) on cyclosporine-induced nephrotoxicity in rats. The results showed that the administration of cyclosporine caused significant alterations in plasma creatinine, blood urea nitrogen, creatinine clearance, kidney injury molecule-1, and neutrophil gelatinase-associated lipocalin levels. However, supplementation with PJ attenuated these alterations and prevented changes in histopathology, lipid peroxidation, and antioxidant enzymes induced by cyclosporine. Therefore, PJ has a protective effect against cyclosporine-induced nephrotoxicity through its antioxidant properties.