期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 1, 页码 238-246出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00811
关键词
exosomes; myeloid derived suppressor cells; N-glycoproteome; cell surface capture
资金
- National Institutes of Health [GM021248, RO1CA115880]
In this report, we use a proteomic strategy to identify glycoproteins on the surface of exosomes derived from myeloid-derived suppressor cells (MDSCs), and then test if selected glycoproteins contribute to exosome-mediated chemotaxis and migration of MDSCs. We report successful modification of a surface chemistry method for use with exosomes and identify 21 surface N-glycoproteins on exosomes released by mouse mammary carcinoma-induced MDSCs. These glycoprotein identities and functionalities are compared with 93 N-linked glycoproteins identified on the surface of the parental cells. As with the lysate proteomes examined previously, the exosome surface N-glycoproteins are primarily a subset of the glycoproteins on the surface of the suppressor cells that released them, with related functions and related potential as therapeutic targets. The don't eat me molecule CD47 and its binding partners thrombospondin-1 (TSP1) and signal regulatory protein alpha (SIRP alpha) were among the surface N-glycoproteins detected. Functional bioassays using antibodies to these three molecules demonstrated that CD47, TSP1, and to a lesser extent SIRPa facilitate exosome-mediated MDSC chemotaxis and migration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据