期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.1023255
关键词
SARS-CoV-2; DNA vaccine; S protein; N protein; mouse model; pPAL; furin
类别
资金
- PTI-Salud Global (CSIC)
- Center for Technological and Industrial Development (CDTI)
- REACT-ANTICIPA-UCM (Comunidad de Madrid)
- European Research Council (Advanced Grant VERDI, ERC2015AdG grant) [694160]
Researchers presented a promising DNA vaccine candidate, pPAL-Sfs + pPAL-N, which demonstrated strong protective effects in mice, fully controlling viral replication and inducing potent humoral and cellular immune responses.
SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we present a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the non-replicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (10(5) PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.
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