期刊
FRONTIERS IN IMMUNOLOGY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2023.1088501
关键词
outer membrane vesicles; Staphylococcus aureus vaccine; SpyCatcher-SpyTag; 'click' display; flexible antigen display; multi-targeting vaccine
类别
In this study, engineered bacterial outer membrane vesicles (OMVs) were used to develop a multitargeting S. aureus click vaccine. The click vaccine, decorated with surface exposed SpyCatcher, can flexibly bind with various SpyTag-fused S. aureus antigens. Compared with antigens mixed with alum adjuvant, the click vaccine induced more potent antigen-specific humoral and Th1-based cellular immune response, providing protection against S. aureus infection in a mouse model.
Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasion strategies developed by S. aureus is thus crucial for its infection control. In this study, we exploited the SpyCatcher-SpyTag system to engineer bacterial outer membrane vesicles (OMVs) for the development of a multitargeting S. aureus click vaccine. We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. The engineered OMVs can flexibly bind with various SpyTag-fused S. aureus antigens to generate an OMV-based click vaccine. Compared with antigens mixed with alum adjuvant, the click vaccine simultaneously induced more potent antigen-specific humoral and Th1-based cellular immune response, which afforded protection against S. aureus Newman lethal challenge in a mouse model. Our study provided a flexible and versatile click vaccine strategy with the potential for fighting against emerging S. aureus clinical isolates.
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