期刊
CELL CYCLE
卷 14, 期 6, 页码 848-856出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2014.1000215
关键词
fission yeast; glucose; Rab GTPase; TOR; TORC2
类别
资金
- National Institute of Health
- JSPS KAKENHI [23870019, 26291024, 23770230, 25440086, 21570198, 26440099]
- Mitsubishi Foundation
- Daiichi Sankyo Foundation of Life Science
- Sagawa Foundation for Promotion of Cancer Research
- Platform for Drug Discovery, Informatics, and Structural Life Science from the MEXT, Japan
- JSPS [23-5163]
- Nara Institute of Science and Technology Global Center of Excellence Program - MEXT
- Nihon University
The Target Of Rapamycin (TOR) is an evolutionarily conserved protein kinase that forms 2 distinct protein complexes referred to as TOR complex 1 (TORC1) and 2 (TORC2). Recent extensive studies have demonstrated that TORC1 is under the control of the small GTPases Rheb and Rag that funnel multiple input signals including those derived from nutritional sources; however, information is scarce as to the regulation of TORC2. A previous study using the model system provided by the fission yeast Schizosaccharomyces pombe identified Ryh1, a Rab-family GTPase, as an activator of TORC2. Here, we show that the nucleotide-binding state of Ryh1 is regulated in response to glucose, mediating this major nutrient signal to TORC2. In glucose-rich growth media, the GTP-bound form of Ryh1 induces TORC2-dependent phosphorylation of Gad8, a downstream target of TORC2 in fission yeast. Upon glucose deprivation, Ryh1 becomes inactive, which turns off the TORC2-Gad8 pathway. During glucose starvation, however, Gad8 phosphorylation by TORC2 gradually recovers independently of Ryh1, implying an additional TORC2 activator that is regulated negatively by glucose. The paired positive and negative regulatory mechanisms may allow fine-tuning of the TORC2-Gad8 pathway, which is essential for growth under glucose-limited environment.
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