High-Throughput DNA Analysis Platform Based on an Optofluidic Ring Resonator Laser

We demonstrate an optofluidic laser using DNA microdroplets, an intercalating dye, and a glass capillary ring resonator. Only the target DNA emits the laser signal, while the non-target DNA, including those with single-base mismatches, exhibits zero optical signals. Subsequently, a single laser emission detected within a few milliseconds can distinguish the target DNA from others, allowing for truly digital DNA sequence analysis from an optical point of view. In addition, the microdroplets could be individually investigated using our optofluidic system. These advances in laser-based DNA analysis make our proposed scheme a promising, rapid, and high-throughput DNA sequence analysis platform.

Automated summary

We present a novel optofluidic laser that utilizes DNA microdroplets, an intercalating dye, and a glass capillary ring resonator. The laser signal emitted from the target DNA allows for accurate and rapid DNA sequence analysis from an optical standpoint, with the capability to distinguish between target DNA and non-target DNA with single-base mismatches.