Draft genome sequence of Streptomyces sp. KD18, isolated from industrial soil

The present study scrutinizes the presence of Streptomyces strains in the soil sample collected from industrial area of Bahadurgarh (Haryana) India. The morphological approach manifested the isolated strain belong to Streptomyces species and named as Streptomyces sp. KD18. Sequencing of Streptomyces sp. KD18 genome was performed by Illumina Nextseq500 platform. 65 contigs were generated via SPAdes v3.11.1 and harboured genome size of 7.2 Mb. AntiSMASH server revealed the presence of 25 biosynthetic gene clusters in KD18 genome where BGC of lipstatin was of more interest from industrial and pharmaceutical purpose. The draft genome sequence represented via ANI values claimed that the KD18 strain belongs to Streptomyces toxytricini and finally named as S. toxytricini KD18. The LC-MS analysis of the extracted metabolite confirmed the production of lipstatin. The genome sequence data have been deposited to NCBI under the accession number of GCA_014748315.1.

Automated summary

This study examined the presence of Streptomyces strains in a soil sample collected from an industrial area in Bahadurgarh, Haryana, India. Morphological analysis confirmed that the isolated strain belonged to the species Streptomyces, named Streptomyces sp. KD18. Genome sequencing of Streptomyces sp. KD18 was performed using the Illumina Nextseq500 platform, generating 65 contigs with a genome size of 7.2 Mb. The presence of 25 biosynthetic gene clusters, including the lipstatin BGC, was revealed by the AntiSMASH server. The strain KD18 was identified as S. toxytricini based on ANI values. LC-MS analysis confirmed the production of lipstatin. The genome sequence data has been deposited to NCBI under the accession number GCA_014748315.1.