Genetic diversity and pathotype profiling of Xanthomonas oryzae pv. oryzae isolates from diverse rice growing ecosystems of Karnataka state of India

Bacterial leaf blight (BLB) disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the signi-ficant constraints for rice production. A study was carried out to analyze the virulence, genetic diversity, and population structure of Xoo isolates collected from different rice ecosystems in Karnataka, India. The taxonomic identity of all 54 iso-lates was confirmed using a Xoo-specific PCR assay. The virulence assay on a set of twenty-eight near-isogenic differentials identified nine pathotype groups (KPthX-1 to KPthX-9). Among them, KPthX-6 was highly virulent, whereas KPthx-2 was less virulent. Differentials with four and five resistance (R) genes (Xa4 + xa5 + xa13 + Xa21, Xa4 + Xa7 + xa13 + Xa21, and Xa4 + xa5 + Xa7 + xa13 + Xa21) were effective against all the pathotypes. Further, genetic diversity was deduced using a universal marker set, JEL-1 and JEL-2, complementary to a conserved repetitive sequence IS1112. The amplicon size and numbers varied from 100 bp to 3 Kbp and 1 to 25. Based on the amplicon counts, Jaccard's co-efficient and phylogenetic analyses were carried out and categorized the 54 isolates into three clusters (I to III). Cluster II contains the maximum number of isolates (27), followed by cluster I (23 isolates) and cluster III (one isolate). The structure analysis categorized the isolates into five subgroups, viz SG1, SG2, SG3, SG4, and SG5 indicate the existence of five subpopulations. The present study has identified the genetic and virulence diversity of the Xoo population and reported the effective R gene/s for different rice ecosystems, which can be used in the breeding program for the eco-specific management of BLB of rice.

Automated summary

This study analyzed the virulence, genetic diversity, and population structure of Xanthomonas oryzae pv. oryzae (Xoo) isolates collected from different rice ecosystems in Karnataka, India. Nine pathotype groups were identified, with KPthX-6 being highly virulent and KPthx-2 being less virulent. Resistance genes Xa4, xa5, xa13, and Xa21 were effective against all pathotypes. The isolates were categorized into three clusters and five subgroups based on genetic diversity. The study provides valuable information for the eco-specific management of bacterial leaf blight (BLB) of rice.