期刊
PEERJ
卷 10, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.14231
关键词
Silver nanoparticles; Shrimp; Aquaculture; Chronic toxicity; WSSV; AgNP; Argovit; Litopenaeus vannamei; Silver fed; White spot syndrome virus
资金
- National Polytechnic Institute, through the research and postgraduate secretariat
- CONACyT [SIP20201761]
- Russian Science Foundation [SIP20201661]
- [SIP20201513]
- [SIP20201543]
- [258607]
- [22-13-20032]
The application of silver nanoparticles as feed additives for shrimp has shown potential in preventing WSSV infection. However, it negatively affects the transcriptional responses of immune-related genes.
In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 (R)) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feedAgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feedAgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据